Abstract:

Objective  To observe the activation of microglia and the photoreceptor cell apoptosis of mice induced by β-chemokines in vitro.  Design  Experimental study. Participants β-chemokine, BV-2 cells of mice, 661w cells of mice. Methods Activation of BV-2 cells were studied when β-chemokines (RANTES, MIP-1αandMIP-1β) were added. The cell death of 661w cells was observed when the supernatant of the activated BV-2 cells was added into the 661w cells. The 661w cell apoptosis was also studied when the β-chemokines were directly added. Met-RANTES (the inhibitor of β-chemokine) was added before hand and above markers were studied. Main Outcome Measures Calcium influx, ROS, NO, TUNEL positive cells. Results The calcium influxesas well as release of ROS, NO by the BV-2 cells was increased (P<0.01) after adding β-chemokine.The 661w cell death was elevated by 30% after adding the supernatant of activated microglia. Above markers can be inhibited by the β-chemokine inhibitors(ROS P<0.01, NO P=0.0187).There was no change when adding β-chemokine into 661w cells directly.  Conclusions β-chemokines can activate microglia, which induce photoreceptor cell death by release of ROS or NO. Photoreceptor degeneration of RP mice may be protected by inhibition of β-chemokine inhibitors. (Ophthalmol CHN, 2017, 26: 270-275)

Key words: β-chemokine, microglia, photoreceptor; , BV-2 cells, 661wcells